Association of T۲۲۲۹/C Exon ۱۲ Polymorphisms of Thyroid Peroxidase Gene with Anti-TPO Levels in Tehran Population

Background and Objective: Antibody secretion in human may be the result of the changes in protein structure. Probably these changes in protein structure or polymorphism in human thyroid peroxidase (TPO) gene is the reason for presence of the anti TPO. In this study, we examined the association of T۲۲۲۹/C exon ۱۲ polymorphism of TPO gene in respect to anti-TPO level. Materials and Methods: In this study ۱۶۸ individuals (۴۷±۲ years) were selected as case and control groups based on anti-TPO titer above and below ۱۰۰ IU/L. PCR-RFLP (polymerase chain reaction- restriction fragment length polymorphism) was used to amplify the segment of exon ۱۲ polymorphism. Results: In exon ۱۲ the allele frequencies were ۰.۸۶۹۸ for C allele and ۰.۱۳۰۱ for T allele and there was no significant association between this polymorphism and anti-TPO level (CC=۱۲۷.۵± ۳۰۸ IU/ml vs. TT=۱۲۶±۲۲۴ IU/ml). Conclusion: This study indicated that there is no significant association between anti-TPO levels and T۲۲۲۹/C exon ۱۲ polymorphisms. Meanwhile, selected SNP of exon۱۲ directly has no effect on anti-TPO levels.

کلیدواژه ها:

Thyroid Peroxidase Polymorphism ، Polymerase Chain Reaction- Restriction Fragment Length Polymorphism