The Study of Effects of Insulin and Ascorbic Acid on Bcl-۲ Family Expression in Hippocampus of Streptozotocin -Induced Diabetic Rats

سال انتشار: 1386
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 207

فایل این مقاله در 15 صفحه با فرمت PDF قابل دریافت می باشد

این مقاله در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_ZUMS-15-60_001

تاریخ نمایه سازی: 11 اردیبهشت 1400

چکیده مقاله:

Background and objective: Diabetes is a metabolic disorder that has been shown to adversely affect both the central and peripheral nervous system by increasing basal neuronal apoptosis. Since Bcl-۲ protein family is considered to play a key role in the regulation of apoptosis, in the present study we have examined the effects of insulin and ascorbic acid on expression of Bcl-۲ family members including Bax (pro-apoptotic) and Bcl-۲ and Bcl-xL (anti-apoptotic) on hippocampus of STZ-induced diabetic rats. Materials and Methods: Five groups of six Wistar rats including one control group (C) and four diabetic groups (D, I, AA and I+AA) were used in this study. Diabetes was induced by injection of ۶۰ mg/kg STZ (IP). After six weeks, rats in group I were treated with insulin (۴-۶ U/kg/day Sc), rats in group AA were treated with ascorbic acid (۲۰۰ mg/kg/day IP) and rats in group I+AA were treated with equal dosage of both insulin and ascorbic acid for two weeks. Rats in group D were treated with normal saline and considered as diabetic control group. Two weeks after treatment, expression of Bcl-۲, Bcl-xL and Bax genes were measured at both mRNA and protein levels. Results: In diabetic control rats (group D), Bax increased whereas Bcl-۲ and Bcl-xL decreased at both mRNA and protein levels compared to group C (P<۰.۰۱, P<۰.۰۰۱ respectively). Interestingly, treatment with insulin (group I), ascorbic acid (group AA) and insulin plus ascorbic acid (group I+AA) could reverse these changes both at mRNA and protein levels (p<۰.۰۰۱ for I and AA+I groups, p<۰.۰۵ (Bcl-۲) and p<۰.۰۱ (Bcl-xL) for AA group). Conclusion: It is concluded that insulin and ascorbic acid alone or together can inhibit apoptosis in STZ-induced diabetic ratschr('۳۹') hippocampus through increasing the ratio of Bcl-۲/Bax and Bcl-xL/Bax expressions. We suggest that inhibition of apoptosis may prevent cognitive dysfunctions induced by hippocampal damage in diabetic patients as well. In addition, further experimental studies will need to be performed to confirm such effects.

نویسندگان

ایرج جعفری انارکولی

دانشجوی دکترای تخصصی علوم تشریحی، گروه علوم تشریحی و واحد تحقیقات علوم اعصاب، دانشگاه علوم پزشکی مشهد، مربی دانشگاه علوم پزشکی زنجان

مجتبی سنکیان

دکترای تخصصی ایمونولوژی، استادیار مرکز تحقیقات و گروه ایمونولوژی، دانشگاه علوم پزشکی مشهد

شهریار احمدپور

دانشجوی دکترای تخصصی علوم تشریحی، گروه علوم تشریحی و واحد تحقیقات علوم اعصاب، دانشگاه علوم پزشکی مشهد

حسین حقیر

دکترای تخصصی علوم تشریحی، دانشیار گروه علوم تشریحی و واحد تحقیقات علوم اعصاب، دانشگاه علوم پزشکی مشهد

شکوفه بنکداران

فوق تخصص غدد، استادیار گروه داخلی و مرکز تحقیقات غدد بیمارستان قائم، دانشگاه علوم پزشکی مشهد

عیدالرضا وارسته

Immuno-Biochemistry Lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran