Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 367

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شناسه ملی سند علمی:

JR_IJBMS-24-4_003

تاریخ نمایه سازی: 6 اردیبهشت 1400

چکیده مقاله:

Objective(s): Strain subtyping is an important epidemiological tool to trace contamination, determine clonal relationships between different strains, and the cause of outbreaks. Current subtyping methods, however, yield less than optimal subtype discrimination. Pulsed-field gel electrophoresis is the gold standard method for Escherichia coli and Multiple-Locus Variable-number tandem repeat Analysis is a rapid PCR-based method. The purpose of this study was to evaluate MLVA and PFGE methods for subtyping β -lactamase-producing E. coli strains isolated from urinary tract infections.Materials and Methods: Overall, ۲۳۰ E. coli isolates from patients with urinary tract infections were examined for antimicrobial susceptibility testing.   ۱۰-loci and ۷-loci MLVA and PFGE methods were used for molecular typing of β -lactamase-producing E. coli isolates. Results: Out of ۲۳۰ isolates, ۱۳۰ (۵۶.۵%) β -lactamase-producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of ۰.۴۸ and ۰.۵۴ for ۷-loci and ۱۰-loci MLVA, respectively.  The discriminatory power of PFGE showed a value of ۰.۸۷. The discordance between the methods was high. Conclusion: Our study showed that PFGE is more discriminatory than MVLA.  MLVA is a PCR- based method and can generate unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA based on geographical region.

کلیدواژه ها:

Beta ، lactamase Escherichia coli Molecular typing Pulsed ، field gel electrophoresis VNTR

نویسندگان

Alireza Dolatyar Dehkharghani

Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Setareh Haghighat

Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Marjan Rahnamaye-Farzami

Department of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, Iran

Masoumeh Douraghi

Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Mohammad Rahbar

Department of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, Iran

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