Application of Gold Core-Shell Magnetic Nanoparticles as an Immunosensor for the Detection of Vibrio cholerae

سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 201

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شناسه ملی سند علمی:

JR_JABR-8-1_011

تاریخ نمایه سازی: 1 اردیبهشت 1400

چکیده مقاله:

Introduction: For rapid and sensitive detection of Vibrio cholerae an accurate assay is needed. In the present study, a gold-coated magnetic nanoparticle (GMNP) was investigated for the detection of V. cholerae. Materials and Methods: GMNPs were synthesized and functionalized by 11-mercapto-undecanoic acid (MUA) as a linker for immobilization of IgG against V. cholerae OmpW antigen. In the next step, IgG was coupled with carboxylic group of MUA using EDC / NHS and the IgG/GMNPs nanocomposite created and finally, the bacterium was detected in a sandwich model ELISA. Results: The IgG/GMNPs nanocomposite could detect V.cholerae in a concentration range from 2.5×10-2 to 1.5 × 105 N/ml (number of V.cholerae per ml). The correlation coefficient was 0.99 and the detection limit was 16 N/ml. Conclusions: In this study, the procedure of antibody immobilization on magnetic nanoparticles was designed. So that, by using magnetic nanoparticles, the pre-concentration as a time-consuming step was removed and the sensitivity of V. cholerae determination was increased. Also, this method can be extended to detect other microorganisms.

نویسندگان

Jamal Rashidiani

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Khadijeh Eskandari

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Reza Ranjbar

Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Hamid Kooshki

Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Davoud Afshar

Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

Farhad Safarpoor Dehkordi

Halal Research Center of IRI, FDA, Tehran, Iran