The effect of TiO2 nanoparticles with different coating on the structure of DNA

Over the past few years, nanoparticles (NPs) have become more and more significant for applications in biomedicine and bio-pharmacy.1Among the semiconductor nanoparticles, titanium dioxide nanoparticles (TiO2NPs) has been utilized most broadly in the nanotechnology and medical industry due to its anti-rust and anti-tumor activities. It is currently believed that TiO2NPs could be used as a medical agent.2,3 Due to the advantages of TiO2NPs in nanotechnology, the aim of this study is to evaluate the effects of ethylene glycol and ethanol coating on TiO2NPs interaction with DNA by fluorescence spectroscopy, circular dichroism (CD) spectroscopy and zeta potential studies. The fluorescence data and binding constants showed that ethylene glycol-coated TiO2NPs could interact with DNA more strongly and make structural changes compare with ethanol-coated TiO2NPs. CD studies and zeta-potential analysis also confirmed the structural variations of DNA after interaction with ethylene glycol-coated TiO2NPs strongly. Totally, the interaction of ethylene glycol-coated TiO2NPs with DNA generated distinct DNA conformation variations. The results obtained from this study proffer a good strategy for designing new drugs and standardization in nanotechnology.

کلیدواژه ها:

Coated-Nanoparticles ، DNA ، Ethylene glycol ، Ethanol ، Titanium Dioxide Nanoparticles.