Labeled peptide synthesis: FAM-YYYYRRRR and study of its binding to para-sulfonatocalix[4]arene supramolecule as a pharmaceutical approach

In recent decades, with the development of drug delivery, the synthesis of peptides in the solid phase has become important. Hence, the peptide FAM-Y4R4 was synthesized, consisting of four arginine (R) amino acids and four tyrosine (Y), and end capping with fluorescein dye. After separation and purification of the synthesized peptide (FAM-YYYYRRRR) by HPLC, a supramolecule para-sulfonatocaliX[4]arene (CX4), also known as the molecular basket, was connected to peptide. To calculate the constant binding of the labeled peptide to supramolecule, it can be measured by fluorimeter and obtain a constant binding. The purpose of this study was to estimate the amount of connection the branched chain of arginine to the CX4. It is expected to change from positive to negative, peptide CX4 to and increase the pathway of cell reabsorption and its permeability to eukaryotic cells. By binding the peptides to a fluorescence dye, it can also be evaluated in the cell for binding and transferring it. Supramolecules, known as carriers of smaller molecules, also have the potential to bind to peptides to transfer drugs into cells without causing toxicity.

کلیدواژه ها:

Peptide synthesis ، Supramolecular connection ، Binding constant ، Fluorimeter