Construction of Alginat Cysteine Polymeric Based Nano Carries for Etoposide in Cancer therapy

Alginate is a naturally occurring, anionic copolymer of guluronic and mannuronic acids and is widely used in the bio encapsulation of drugs, proteins and cells. It is biodegradable, biocompatible, non-toxic properties; and has the ability to form a gel in the presence of divalent cations such as calcium1. To improve its properties, a variety of synthetic and natural polymers have been proposed for blending with alginate2, 3. In this study, etoposide, a typical chemotherapeutic agent, was selected as the model drug to evaluate of cysteine-modified sodium alginate nanoparticles to load drug. The structure of nanoparticles was elucidated by FTIR. The shape, and morphology were determined by SEM. The mean size, PDI and ZP of the obtained drug loaded nanoparticles were reported to be 355.4 nm, 0.309 and -27.7 mV, respectively. The in-vitro drug release was studied by using UV–Visible spectrophotometer at acidic environment and physiological pH and sustained release compared to the ETO alone. It was found that ETO drug is released much faster in pH 5.0 than in the pH 7.4. In addition, the cytotoxicity of the created nanoparticles was performed by using LDH assay analysis which showed that ETO loaded nanoparticles SA/Cys/ETO were toxic to MCF-7 cell line.

کلیدواژه ها:

Polymeric carbohydrate ، L-Cysteine ، Anticancer drug ، Drug delivery