Isolation and Identification of A Novel Strain of Acetobacter ghanensis KBMNS-IAUF-6 from Banana Fruit, Resistant to High Temperature and Ethanol Concentration
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 270
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شناسه ملی سند علمی:
JR_IJMM-13-4_002
تاریخ نمایه سازی: 10 اسفند 1399
چکیده مقاله:
Background and Aims: The use of ethanol- temperature-resistant Acetobacter strains are of importance in vinegar industry. The aim of this study was to isolate and identify high temperature and ethanol resistant Acetobacter strains as starter for production of vinegar.
Materials and Methods: The banana alcoholic extract was transferred to the fermenter medium and after 24 h of incubation at 30°C, colonies with transparent zone were purified as acetic acid bacteria and examined macroscopically and microscopically. The resistance to high temperature in constant ethanol and time as well as resistance to high ethanol in constant temperature and time were investigated.
Results: The studies of AAB isolate grown in the Carr medium showed that it was an Acetobacter strain. According to the single-factor optimization, this species was able to grow in a Carr medium containing 9% ethanol at 40°C after 72 h.
Conclusion: This is the first report of an AAB isolation from banana in Iran. This bacterium, as a new resistant strain to high levels of ethanol and temperature, was identified as Acetobacter ghanensis KBMNS-IAUF-6 and its 16s-rDNA sequence was deposited in GenBank, NCBI, under the accession number of MK968570. This new strain can be suggested as a high temperature and ethanol resistant strain for producing banana vinegar on a semi-industrial and industrial scale.
کلیدواژه ها:
Acetic acid bacteria ، Acetobacter ghanensis ، Industrial and food biotechnology ، Temperature and ethanol resistant strain ، استوباکتر گاننسیس ، باکتریهای مولد اسیداستیک ، بیوتکنولوژی صنعتی و غذایی ، سویه مقاوم به دما و اتانول
نویسندگان
Keivan Beheshti-Maal
Associate Professor of Microbiology, Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Noushin Shafiee
M.Sc. of Microbiology, Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
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