Extraction of Abscisic acid, Gibberellic acid and Auxin from Sargassum sp. and Gracilaria corticata Macroalgaes for Use in Algae Liquid Fertilizer in Bushehr Port

In the present study, to extract three regulating plant growth hormones, Abscisic acid, Gibberellic acid and Auxin from Sargassum sp. and Gracilaria corticata on the shores of Bushehr, sampling was performed from January ۲۰۱۵ to November ۲۰۱۶. Macroalgae biomass and environmental factors measured and recorded. For extraction of ABA, IAA and GA۳, the algae transferred to the laboratory. After the end of the extraction process with the help of the HPLC method, these hormones were separated and identified by standard injections. The results of ABA extraction indicated that minimum of this hormone in both algae is ۰% and maximum was ۲.۰۶۶۷% in Sargassum sp. in September and ۶.۶۵۸۳% for Gracilaria. The results of GA۳ extraction also showed that minimum was ۰% and maximum was ۵.۸۵۶۱% in Sargassum in July. In Gracilaria minimum obtained in November and it was ۰.۸۹۰۷% and Maximum obtained in May and it was ۸.۴۴۶۷%. Auxin extraction results also showed that minimum in both algae was ۰% and maximum in May and equalled to ۱.۴۱۱۳% in Sargassum. In Gracilaria, this amount was ۱.۸۵۱% in January. In two algae species identified, the maximum biomass related to brown algae Sargassum in January (۶۷۹g/m۲) and the minimum biomass was in September (۲۰.۶۷ g/m۲). Concerning red algae Gracilaria, the maximum biomass was in March (۴۲۳/۴۳۳ g/m۲), and in November (۱۵۸g/m۲) it had the minimum biomass. The results of ANOVA and Chi-square statistical tests showed a significant difference between six months (P <۰.۰۵). Generally, a protocol designed to extract growth-regulating hormones. Hormones extracted in this study used in algae liquid. Due to the very high price of plant hormones and the necessity of using these hormones in algae liquid fertilizers and algae extracts, this study might be a useful source for extraction of a variety of hormones from these algae.

کلیدواژه ها:

Abscisic acid ، Gibberellic acid ، Auxin ، Sargassum ، Gracilaria