Effect of temperature, phosphorous and light on growth of Oscillatoria agardhii

In this study the sampling operation has been done in Gorgan‟s gulf water in the beaches of Caspian Sea and alga pile Oscillatoria agardhii was separated from the water with algae sample by pipet and hookpang method. Then they have been kept on the agar plate for the first culture. O. agardhii was obtained in medium Z8-N with limited mass. The different density of phosphors (100 and 200μg/lit) and temperature 25-30 and 30-35 oC in optic intensities 3000, 3500 and 4000 deluxe with different light alternation (10/14 and 14/10 L/D) was grown. The results showed that the most rates of cell numbers for the period 10/14 and temperature of 25-30 and 30-35 related to the Z8-N medium on the tenth day, which was 3.46 and 3.42 respectively. For light period 14/10 and temperature of 25-30 in 3 medium, the highest cell amount was on the fourth day. The weight of alga in Z8-N medium and in 25-30 temperature and in light period 10/14 in comparison with the other two medium had the most rates, which was 0.78 and 0.77 respectively. In light period 10/14, it was found that the highest rate of alga‟s weight in the fourth day in all three medium and was 3/57. Additive process in alga‟s cell time division showed in all three medium at 25-30, 30-35 °C, 10/14 light period, and at the 25-35 °C, 14/10 light period showed the highest rate in 200 medium on the fourth day. The highest growth rate in comparison with three other mediums related to Z8-N medium and the least related to 100 medium on the fourth day. The most rate of cell division in 30-35°C, 10/14 light period was seen in Z8-N medium and the least rate was in 100 medium

کلیدواژه ها:

Oscillatoria agardhii ، light period ، temperature ، medium ، darkness and lightness