Effects of Rheum ribes on paraoxonase1 activity in hyperlipidemia rats

Introduction & objectives: Paraoxonese1 (PON1) is an enzyme associated with high density lipoprotein (HDL) in serum, with aryl esterase and paraoxonase activity. The antioxidant properties of this enzyme protect the oxidation of low density lipoprotein (LDL). Certain antioxidant compounds and medical plants, such as Rheum ribes, can protect the LDL from oxidation. In this study, we evaluated the effects of Rheum ribes on the PON1 enzyme in hyperlipidemic and hypercholesterolemia rats and investigated the molecular dynamic effects of Cinamic , Glucogallin and Quercetin (the most compounds of Rheum ribes) On PON1 activity.Materials & Methods: We used hydro-alcoholic extract of Rheum ribes in hyperlipidemia and hypercholesterolemia rats for the demonstration of the PON1 enzyme activity by aryl esterase properties and the PON1 gene expression. Then, we demonstrated the molecular dynamic (MD) effects of their compounds (Cinamic , Glucogallin and Quercetin) on PON1 via simulation studies using the AutoDockv:4.0.0 and the Gromacs 2018 software.Results: Rheum ribes reduced the serum triglycerides (TG) and total cholesterol (TC), and increased the PON1 serum aryl esterase activity, however, had no significant effect on the PON1 gene expression. The simulation and the MD results proved that among all the compounds, Glucogallin and Quercetin have more tendencies to bind to the enzyme. However, the radius of gyration (RG), increased when three compounds bind to PON1.Conclusion: Rheum ribes can increase the PON1 activity in serum, without increasing the gene expression. According to simulation and MD studies, Glucogallin and Quercetin approximately had the most important for increasing the PON1 activity.

کلیدواژه ها:

Rheum ribes ، PON1 enzyme ، Molecular dynam