Co-Culture of mesenchymal stem cells with endothelial progenitor cells inside alginate-gelatin microspheres promoted angiogenic potential

Introduction: Here, we investigated the angiogenic potential of endothelial progenitor cells juxtaposed with mesenchymal stem cells inside alginate-gelatin microspheres .Methods: Three encapsulated groups were allocated including EPCs, MSCs, EPCs+MSCs. Cells were encapsulated with a mixture of 1% alginate and 2% gelatin hydrogel. Cell survival was examined by MTT assay. Endothelial differentiation was determined by flow cytometry and ELISA. Tubulogenesis assay and Ac-Dil-LDL uptake were used to detect functional activity. Cell migration was analyzed by Transwell insert and gelatin zymography analyses. By using real-time PCR, we measured the transcription of Akt and PK1.Results: We found an increase in cell viability in MSCs/SDF-1α microspheres compared to EPCs group (p<0.05). EPC/MSCs co-culture contributed to the increase of CD133+ cells while we found high CD31 levels in MSCs group (p<0.05). Juxtaposition of EPC with MSCs increased tubulogenesis compared to Culture of cells individually .Conclusions: Alginate-gelatin microspheres could alter the angiogenic potential of progenitor cells .

کلیدواژه ها:

Endothelial Progenitor Cells ، Mesenchymal Stem Cells ، Alginate-gelatin Microspheres ، Angiogenesis