Synthesis and fabrication of quercetin-loaded electrospun nanofibrous mats with antioxidant activity for proliferation and stemness preservation of human mesenchymal stem cells

Introduction: An ideal biomaterial in regenerative medicine should be able to regulate the stem cell proliferation without the loss of its pluripotency. quercetin is one of the most important flavone with a wide spectrum of biological functions including anti-inflammatory and anti-oxidant properties. The present study describes the influence of Que-loaded electrospun nanofibers on the regulation of proliferation and stemness preservation of Mesenchymal stem cells (MSCs).Methods: For this purpose, Que -loaded poly (e-caprolactone)/poly (ethylene glycol) (PCL/PEG) electrospun nanofibers were formed via electrospinning procedure and the effective fabrication of these bioactive nanofibers was confirmed by field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared spectroscopy. MSCs were seeded on the electrospun nanofibers and their morphology, viability, and stemness expression were analyzed using FE-SEM, MTT, and qPCR assays after one weeks of incubation, respectively.Results: The results show that MSCs display better adhesion and significantly increased viability on the Que -loaded PCL/PEG nanofibrous mats in relative to the PCL/PEG nanofibers and tissue culture polystyrene. The greater viability of MSCs on Que based electrospun nanofibers was further confirmed by higher expression levels of stemness markers Sox-2 and Nanog.Conclusions: These results reveal that Que-loaded PCL/PEG electrospun nanofibrous can be used to improve cell adhesion and proliferation while synchronously preserving the stemness of MSCs, thus representing a hopeful potential for application in stem cell therapy strategies.

کلیدواژه ها:

quercetin ، Electrospun nanofibers ، Stem cells ، Pluripotency