S14161 as a novel small molecule promoted the apoptosis in A549 cancer cells

Background and objectives: Lung cancer is the second most widely recognized cancer in the world that causes the highest cancer deaths in men. Since most modern cancer therapies are ineffective and have many side effects, effective treatment is therefore needed. Small molecular cases have been identified today in the treatment of cancer with little side effects. One of these small molecules is the S14161 that is PI3K inhibitor. Therefore, in the present study, the effect of apoptotic S14161 small molecule in A549 cell line was investigated.Material and Methods: Cell cytotoxicity of S14161 small molecule at concentrations of 2, 5, 10, 20, and 40 (mM) was investigated by MTT technique and Bcl2, Bax gene expression was measured by real-time PCR method. One-way ANOVA was used for statistical analysis.Results: In the MTT assay, the IC50 of S14161 small molecule was determined in the A549 cell line, 10 Mm, which showed a dose-dependent cytotoxic effect (p <0.05). The results of qRT-PCR showed an increase in Bax gene expression and a decrease in Bcl2 gene expression in A549 cells within 24-hour treatment with S14161 small molecule, which induces apoptosis.Conclusion: S14161 small molecule by inducing PI3K/AKT pathway can induce apoptosis in A549 cells and can be a good candidate for cancer treatment.

کلیدواژه ها:

A549 ، PI3K ، Apoptosis ، S14161