Evaluation of the role of rs3789327 polymorphism in increased risk of multiple sclerosis

Introduction: Multiple sclerosis (MS) is an inflammatory demyelinating and potentially disabling disease of the central nervous system with varied clinical appearances and heterogeneous histopathological features. Today, it has been found that alterations in single nucleotide sequences (polymorphisms) are associated with predisposing or resistance to diseases. The past studies have shown that genetic alterations in key regulators of circadian rhythm, such as the ARNTL gene, may increase the risk of MS. The aim of this study was to evaluate the frequency and association of rs3789327 polymorphism in ARNTL gene with multiple sclerosis in MS patients compared to healthy individuals in Khuzestan population.Materials and Methods: Sixty patients with multiple sclerosis referred to the MS Community Center of Isfahan as case group and 50 healthy subjects as control group was selected. Then, 3 cc whole blood were taken and shed in EDTA tubes and were stored in -20 ° C until DNA extractions. The genotyping for rs3789327 polymorphism was evaluated by PCR-Sequencing.Results: In this study, AA genotype was 20% in patients and 27% in the control group. AG genotype in patients was 35% and in healthy subjects 40% and GG genotype of this polymorphism was 45% in patients and 33% in healthy controls. The prevalence none of the genotypes were statistically significant in the two groups (P> 0.05).Conclusion: The results showed that there is no significant relationship between rs3789327 polymorphism in the ARNTL gene and susceptibility to multiple sclerosis in studying population.

کلیدواژه ها:

Multiple Sclerosis ، ARNTL gene ، Polymorphism