Determination of Antioxidant Potential, Phenolic Content, Flavonoid Content and Antitumor Effect of Trachyspermum ammi seed Essential Oil on Breast Cancer Cell lines MDA-MB-231, MDA-MB-468, Mcf7

Background: Cancer is one of the leading causes of mortality worldwide and breast cancer is the second most common cancer among people. The persistent presence of saturated free radicals in the natural cells of the mutant causes the cancer to weaken by weakening the antioxidant system. Plants contain antioxidants that reduce the incidence and progression of cancers by reviving free radicals. The aim of this study was to determine the phenolic content, flavonoid content, antioxidant power and antitumor effect of essential oil of Trachyspermum ammi seed.Materials and Methods: In this in vitro experimental study, phenolic content of essential oil of Trachyspermum ammi seed using Folin's reagent, flavonoid content of this extract using Aluminum Chloride (AlCl3), essential oil antioxidant activity The use of two methods (DPPH) of 2,2-diphenyl-1-picrylhydrazyl and Ferric reducing ability of plasma (FRAP) at different concentrations were evaluated, and the antitumor effects of this essential oil by MTT assay on MDA- cell lines. MDA-MB 231, MDA MB-468, Mcf7 were evaluated.Results: Phenolic content of the essential oil of Trachyspermum ammi seed 429/63±25/52 μgr GAE/mgr dw, Flavonoid content of this essential oil 467/17±10/75 μgr EQ/mgr dw, monofluorescence essential oil Adult seed was obtained 374/40±41/68 μMFe2+/gr And for inhibition of DPPH free radicals, IC50 of 4934 μg/ml was obtained.The mean IC50 was obtained for Mcf7 cell line 222.3,MDA-MB-468 334/03,MDA-MB-231 291/03μg/ml.Conclusion: Trachyspermum ammi seed with remarkable antioxidant power at high concentrations are likely to be able to inhibit free radicals and can maintain cancer balance by maintaining antioxidant system and cell homeostasis. Prevention. It is recommended that in future animal studies or clinical trials, the therapeutic effect of this plant on the reduction of tumor growth and proliferation of cancer cells be investigated.

کلیدواژه ها:

Extract ، Antioxidant ، Phenol ، Flavonoid ، DPPH ، FRAP ، Breast Cancer ، MTT