Molecular identification of Staphylococcus epidermidis and Staphylococcus aureus in children and adults suspected of sepsis in Rafsanjan city

Introduction & Objectives: Septicemia due to numerous health risks is considered a medical emergency. bacterial infection is one of the most common infections. some bacterial agents that cause bloodstream infections include Staphylococcus aureus, Pseudomonas aeruginosa, klebsiella pneumoniae, Acinetobacter, Escherichia coli and Enterobacter. Blood infection caused by opportunistic bacteria such as Coagulase-negative staphylococci is one of the major problems of hospitalized and immunosuppressive patients. Since the 1980s, gram-positive microorganisms, especially Staphylococcus aureus, have persisted as a major cause of nosocomial infections. In this study, molecular identification of Staphylococcus epidermidis and Staphylococcus aureus in blood samples suspected of sepsis was performed by PCR method.Materials & Methods: A total of 209 venous blood samples were collected from suspected sepsis patients in Rafsanjan city Medical Laboratories. 100 μl of collected blood was used for culture in blood agar and then in nutrient broth. DNA was extracted by DNG-Plus kit and PCR test was used to detect Staphylococcus epidermidis and Staphylococcus aureus.Results: 55 samples of 209 were grown in liquid culture media. From PCR results, 44 samples were identified as infected with Staphylococcus epidermidis and 11 samples were infected with Staphylococcus aureus.Conclusion: PCR test is a fast and sensitive method that is preferable to conventional laboratory for definitive detection of bacterial sepsis, which is very valuable to both physicians and patients. This study showed that compared to other researches, the prevalence of these bacteria has increased in patients from Rafsanjan city.

کلیدواژه ها:

Staphylococcus epidermidis ، Staphylococcus aureus ، Sepsis ، PCR