Wound healing effects of ZnO nanocomposite containing Alchemilla extract in rats

Introduction & Objectives: Wound healing still remains a challenging clinical problem for which efficient wound management is necessary. there is a desire for novel strategies to achieve expeditious wound healing because of the enormous financial burden worldwide. in recent decades, researchers have focused on the development of nanotechnology. drug delivery systems are expanding to meet the specific goal and increasing the burden of treatment using nanotechnology. to minimize wound infections, they often use disinfectants to coat the wound. The aim of this study is investigation of ZnO and ZnO containing Alchemilla extract effects on wound healing. Materials and Methods: In the present study, ZnO metallic nanoparticle (NP) via Alchemilla extract (Ext) and one without Alchemilla namely: ZnO/Ext and ZnO (w) were prepared by microwave method and assessed for their wound healing activity on excision experimental model of wounds in rats. The developed nanocomposites have been characterized by physico-chemical methods such as; X-ray diffraction (XRD), Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR). The wounded animals dedicated to ZnO, ZnO/Ext and control groups. In all groups, the wounds were treated by prepared samples for 3 weeks. Every seven days, after measuring decreasing rate of wound size, three samples from each group were separated for histopathological analysis.Results: The in vivo evaluation reveals that the ZnO/Ext nanocomposite dressing enhances the wound healing and promotes re-epithelialization.Conclusion: The results showed that the ZnO/Ext is significantly the most effective material in comparison with ZnO (W) and control.

کلیدواژه ها:

Nano-biotechnology ، wound healing ، zinc oxide ، Alchemilla