The effect of Platelet Rich Plasma on proliferation and survival of Adipose –Derived Stem Cells in Fibrin and Alginate scaffolds

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 223

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شناسه ملی سند علمی:

ICIBS01_159

تاریخ نمایه سازی: 2 آذر 1399

چکیده مقاله:

Background: Today, stem cells are the best candidate for cell therapy and tissue engineering. In this study, proliferation and viability of ADSCs affected by PRP (Platelet Rich Plasma) in Alginate and Fibrin scaffolds were evaluated and compared.Materials and Methods: ADSCs in third passage, were encapsulated in fibrin and alginate scaffolds and affected by medium with PRP10% or FBS10%. Then cell viability evaluated by MTT assay and Trypan blue staining. The apoptosis was assessed by Annexin V-FITC Kit on fourth and eighth days. Data analyzed by ANOVA (one-way).Results: The MTT and Trypan blue staining results showed that the rate of viability in fibrin with PRP was higher than other groups significantly (P<0.05). Percentage of early apoptotic cells in fibrin with PRP was significantly lower than other group (P<0.01). Late apoptotic cells were not significant different between the groups.Discussion: The PRP has several advantages compared with recombinant growth factors and animal serum and can be considered as suitable supplement for stem cell culturing in compared with FBS.

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نویسندگان

Batool Hashemibeni

Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Marzieh Sadeghian

Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Reihane Seyed Ebrahimi

Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran