Effect of Cartilage ECM on polycaprolactone/Fibrin hybrid scaffold fabricated by salt leaching method

سال انتشار: 1399
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 267

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شناسه ملی سند علمی:

ICIBS01_158

تاریخ نمایه سازی: 2 آذر 1399

چکیده مقاله:

Introduction: The Ideal three dimensional (3-D) scaffold in Cartilage tissue engineering should be biocompatible and biodegradable to enhance cell attachment, adequate mechanical properties and high porosity with suitable interconnected pores to improve cell ingrowth and nutrient transportation. Salt leaching technique include making a solution of polymer in solvent and mix salt particles of a fixed diameter as porogen to produce a homogenous suspension and fabricate a porous structure. The main advantage of using cartilage ECM derivatives as a scaffold is its ability to maintain growth factors. Therefore, such ECM could potentially be used as a carrier for freshly isolated stem cells, with such a construct forming the basis of a single-stage therapy for articular cartilage regeneration.Materials and Methods: In this study, PCL scaffold was prepared with salt leaching method. ECM was mixed with fibrinogen solution in 2% (w/v), 5% (w/v) and 10% (w/v). ADSCs added to ECM/ fibrinogen solution and seed on the PCL scaffolds in different groups that cells inhabited in the scaffold porosity. Then thrombin was added to this complex and allowed to gel. MTT assay, water absorption, scanning electron microscopy and dynamic mechanical analysis were used to characterize the mechanical properties of the resulting scaffolds.Results: data showed that stem cell can proliferate well on PCL/Fibrin scaffold coated with ECM and a PCL salt-leached scaffold had a compressive modulus of 0.2 – 0.5 MPa. ECM 2% group presented a high significant cell viability that was suitable for cell ingrowth compare with ECM 5% and ECM 10%.Conclusions: when the ECM concentration was increased (10% w/v), the structure cannot preserve its primary shape, consequently using of 2% (w/v) ECM embedded in PCL/Fibrin hybrid scaffold is recommended.

نویسندگان

Ali Honarvar

Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Ali Valiani

Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Batool Hashemibeni

Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran