Evaluation the biodistribution of RGD-modified nanoliposomes containing curcumin in rats by HPLC

Introduction: Nanotechnology has many applications in biomedical sciences. Various nano-carriers such as liposomes play a vital role in enhancing the efficacy of drugs and therapies. RGD or (arginine-glycine-aspartic acid) peptide is used to target integrin receptors expressed on cancer cells. In this study, the biological distribution of RGD-modified nano-liposomes containing curcumin was investigated in different tissues of normal rats by HPLC analysis.Method: The nano-liposomes were prepared by lipid thin film hydrolysis. Thirty male Wistar rats were divided into 5 groups of 6. Then, 500 ml of each sample was injected through intraperitoneal (IP) in experimental animals. Group 1: No medication. Group 2: receiving PBS. Group 3: receiving liposome containing curcumin. Group 4: receiving RGD modified liposome containing curcumin and Group 5: receiving curcumin only. The amount of curcumin in different tissues such as blood, liver, kidney, spleen, lung, heart and brain was measured using HPLC analysis after 24 hours of injection. First, different tissues were disrupted using a homogenizer and centrifuged. Then, the amount of curcumin in the supernatant was measured using HPLC analysis.Result: The HPLC results showed that the amount of curcumin in serum tissue was high in the castrated groups (group4> group 3> group 5> group 2 = group 1, and high in liver in group 4> group 5> group 3> group 2 = group 1. It was also high in kidney tissue in group 5 > group 4> group 3> group 2 = group 1. There was not any distribution in other tissues such as heart, lung, spleen and brain tissue.Conclusion: The results showed that the distribution of RGD-modified liposomes containing curcumin was higher in blood, liver and kidney tissues than other groups in other tissues.

کلیدواژه ها:

Curcumin ، bio-distribution ، RGD-modified liposomes