miRNA-mRNA Regulatory Networks of Some Neurotransmitter Receptors in Nervous System Disease

Introduction and Objectives: MicroRNAs are small noncoding RNAs which modulate protein expression and play key roles in the pathways regulating neurogenesis and synaptic plasticity. These small RNAs may be important for the pathophysiology of mental disorders and may affect the efficacy of psychotropic drugs.In this project, we got microRNAs that inhibit some receptors of neurotransmitters and constructed interaction networks of miRNA-mRNA.Materials and Methods: Mirwalk database was applied to get the validated microRNAs which regulate some receptors of neurotransmitters expression (HTR1A, HTR1B, HTR1D, HTR2C, HTR3A, HTR7, GABRB3, GABBR2, ADRA1B, ADRA2B, ADRB3, UBE2D2, GRIN2B, GRIA2, GRIK2, GRM1, GRM3, GRM4, GRM5, GRM6). After that, these data were imported in cytoscape software, and miRNA-mRNA networks were constructed and analyzed. Also, HMDD database was applied to evaluation of relationship between significant miRNAs in the network. Results: Evaluation of miRNA-mRNA interaction networks of neurotransmitters receptors showed that these interaction networks include 253 nodes. MiR-124-3p (degree:7) and miR-335-5p (degree:5) have the most degree in the network. Also, HMDD (the Human microRNA Disease Database version3.2) indicated that expression of miR-124-3p is changed in some Nervous System Diseases such as Depression Disorder, Bipolar Disorder, Schizophrenia, Neurodegenerative Diseases, Alzheimer’s Disease, and Neuroinflammation. Conclusion: Results indicated miR-124-3p is significantly powerful miRNA that regulated series of important receptors genes in the brain. Therefore, miR-124-3p could be considered a perfect target for the treatment of nervous system disease.

کلیدواژه ها:

Interaction network ، microRNA ، neurotransmitter