A study of probability distribution of the single nucleotide variants of rs7579 and rs34713741 inSEPP1 and SELs genes of the colorectal cancer patients compared with a control group of random patients in Isfahan AL-zahra hospital

Introduction: Colorectal cancer is one of the most common cancers worldwide, and according to WHO in 2013, 45 of every 100000 people were develop this cancer. In studying genetic factors that cause this cancer, attention is focused on mutations. Nowadays, extensive and numerous studies are conducted on the relationship between genetic changes such as polymorphisms and the risk for developing various kinds of cancer. Research has shown that some of selenoproteins polymorphisms are also involved in the development of various types of cancer, among which the pivotal role of rs7579 is in the SEPPI and rs34713741 in the SELS genes.Methods: In this research, peripheral blood samples were randomly taken from 60 colorectal cancer patients and 74 healthy people who were matched to the patients with respect to age and gender. The DNA genomes were extracted from the blood samples using kit, and the HRM technique was employed to study two polymorphisms.Results: HRM diagrams related to the polymorphism were analyzed, the genotypes in the case and control groups were then compared, and a significant relationship was observed between frequency distributions A, G alleles of the rs7579 polymorphism (p value = 0.03). In the SELS gene, no significant correlation was found between allele frequency and genotypic distribution in rs34713741 polymorphism and colorectal cancer. (pvalue=0.93).conclusion: Based on results of this study, we can suggest that there is a significant relationship between the rs7579 polymorphism of the SEPPI gene and increased risk for colorectal cancer development. In this study, there was not found any significant relationship between rs34713741 in the SELS gene and colorectal cancer.

کلیدواژه ها:

Colorectal cancer ، SEPPI gene ، SELS gene ، HRM ، polymorphism