The effect of static magnetic field on mouse pre-antral follicle growth and matured oocyte fertilization in vitro

Introduction & Objective: In vitro culture of ovarian follicles is a method to evaluate of folliculogenesis that may be used in assisted reproductive methods. Recent research is aimed to find efficient methods to increase the number of healthy follicles to obtain fertilized oocytes from primary follicles in the culture medium. Our aim this study was to evaluate the oocyte maturation rate during in vitro culture of fresh mouse pre-antral follicles under the static magnetic field (SMF).Materials & Methods: Pre-antral follicles were isolated mechanically from the ovaries of pre-pubertal mice and distributed in to two groups: 1. CFF: Cultured Fresh Follicles 2. CFF + 1mT: Culture Fresh Follicle + 1mT SMF (10 min). The fresh follicles were incubated in 5% carbon dioxide (CO2) at 37◦C for 12 days and the medium was replenished every other day. In this study, Follicle survival, follicle development and oocyte maturation were assessed during in vitro culture in two experimental groups.Results: In the CFF + 1mT group, antrum formation rate was higher comparing to the CFF group, whereas follicle survival rate was similar. In the SMF exposured group had higher oocyte maturation rate than the control group, 2PN formation rate in CFF + 1mT group was higher than in CFF group (P<0.05).Conclusion: It was concluded begin exposed to SMF during in vitro follicle culture leads to an improvement in follicle development and oocyte maturation.

کلیدواژه ها:

Pre-antral follicles ، Static magnetic field