Evaluation of Grape seed extract (GSE) on PTEN and DACT1 tumor suppressor genes expression, cell cycle and apoptosis in ovarian cancer cell line (OVCAR-3) in vitro

Introduction and Objectives: Ovarian cancer is the most common malignancy that leading cause of cancer mortality in women. Grape seed extract (GSE) exhibit anticancer effect against several different types of cancer, we aimed to determine the expression of PTENT and DACT1 tumor suppressor genes using GSE and then investigate the growth rate, proliferation, and apoptosis in ovarian cancer cell line.Materials and Methods: The ovarian cancer cell line (OVCAR-3) was treated with GSE (71 μg/ml) for 24 and 48 h invitro while the untreated group was considered as the control. The cell viability and the apoptosis rate were measured by MTT and flow cytometry, real-time PCR was used to determine expression of PTENT and DACT1 tumor suppressor genes and Downstream genes involved in the cell cycle and apoptosis and protein expression was examined by caspase colorimetric.Results: It was found that treatment of ovarian cancer cells with GSE, increased the expression level of PTENT and DACT1 tumor suppressor genes and also increased caspase3,8 and 9 gene expression(p<0.001). the cell growth inhibited through decrease the expression of cyclinD1 and CDK4 (p<0.001). flow cytometry indicated that GSE increased cell apoptosis in OVCAR-3 cells in a time-dependent manner (p<0.001). also by caspase colorimetric identified that GSE treatment increased caspase-3 protein expression (p<0.001).Conclusions: These results suggest that GSE has anti-cancer effect in ovarian cancer cells by inhibited cell proliferation by inducing cell cycle arrest and also increases apoptosis in these cells. Thus, GSE may be useful as herbal antitumor agent for cancer therapy.

کلیدواژه ها:

Grape seed extract ، ovarian cancer ، tumor suppressor genes ، apoptosis