Expression Analysis of Has-mir-629-5p in Cancerous Tissues from Iranian Patients with Colorectal Cancer

Introduction and Objectives: The incidence and mortality rate of colorectal cancer (CRC) is significant around the globe, which leads to understanding the early molecular events of CRC. During the last decades, there has been tremendous growth in the biology of microRNAs as the potential therapeutic targets in cancer. MicroRNAs are small, non-coding RNAs which regulate gene expression at the post-transcriptional level and they may function as both oncogenes and tumor suppressors. Moreover, they play a vital role in different physiologic processes in the initiation and progression of cancer. Has-miR-629-5p in several cancers was upregulated and play critical roles in tumor progression by various cellular functions. However, the role of miR-629-5p in colorectal cancer remains unclear. Materials and Methods: In this study, we evaluated the expression of miR-629-5p in both 40 cancerous tissue samples and 40 adjacent healthy tissue samples achieved from patients with colorectal cancer. All the RNA molecules extracted from tissue samples by using trizole. cDNA was synthesized by stem-loop RT-PCR technique. The expression of miR-629-5P evaluated by quantitative real-time RT-PCR in both case and control groups. Results: The results showed that the expression levels of miR-629-5p increased noticeably in tumor samples than normal tissues. The statistical analysis was performed via Prism software which showed (p value< 0.05). Conclusions: In overall, our findings illustrated that, miR-629-5p could be used as a promising biomarker for diagnostic and prognostic in different stages and also a potential therapeutic target in colorectal cancer.

کلیدواژه ها:

MicroRNA ، colorectal cancer ، mir-629-5p ، RT-PCR