Mir-30 a master regulator that affects angiogenesis and stemness in melanoma tumor

Objectives: Mir-30 plays a dual role in tumor angiogenesis acting both as a suppressor and promoter of tumor vessel formation. Stemness profile almost often is negatively controlled by Mir-30 decreasing tumor progression. The aim of this study was the evaluation of mir-30 transfection in melanoma tumor angiogenesis and stemness profile.Material and methods: MiR-30a-5p has been transfected into B16-F10 melanoma cells, then cells were injected subcutaneously in to C57BL/6 mice. Then the mice were sacrificed and the vessel density was evaluated by immunohistochemical staining procedure. The extent of nestin and oct-4 expression was evaluated by RTQ-PCR to assess stemness.Results: Vessel density significantly decreased in melanoma mouse models after transfection with miR-30a-5p when compared with control group and scramble as negative control. Mir-30a transfection significantly increased Nestin and oct-4 mRNA expression. Our results offer a significantly different explanation distinct from many other experimental observations suggesting overexpression of miR-30 in melanoma tumor cells increased their self-renewal ability and stemness profile. Conclusion: Overexpression of Mir-30 does not always correlate with desirable outcomes and its overexpression may result in tumor progression and poor prognosis due to increasing stemness state ensuring excess proliferation of tumor cells.