Electrochemical Glucose Biosensor based on Gold-Coated Magnetic Nanoparticle and Investigation of Nanoparticle Effect on Glucose oxidase

A feasible and fast method to fabricate glucose biosensor was developed by covalent attachment of glucose oxidase (GOx) to a gold-coated magne-c nanopar-cle (Fe@Au)[1,2]. The direct electrochemistry Glucose oxidase not contain medioter and worked in air saturated condition for similarity of invivo usage. Glucose oxidase molecules were oxidized with metaperiodate to aldehydes group formation. Gold-coated magnetic nanoparticle was manufactured by cysteamine and used as a carrier for the immobilization of glucose oxidase (GOx). The prepared Fe3O4/GN composite nanopar-cles were about 50nm in diameter and the glucose oxidase was covalently attached to the amine-modified nanoparticles. Themodified electrode showed a quasi-reversible cyclic voltammogram corresponding to the FAD/FADH2 redox couple with a formal potential of -270 mV in 0.1 M phosphate buffer solu-on at pH 6.8. The The linear concentra-on rang of biosensor is 2.4–42 mM and with detec-on limit of 0.51mM at S/N = 3. The life-me of biosensor is more than 2 week.

کلیدواژه ها:

glucose oxidase (GOx) ، gold-coated magnetic nanoparticle (Fe@Au) ، Biosensor