Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation

سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 482

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شناسه ملی سند علمی:

JR_IJP-15-3_009

تاریخ نمایه سازی: 31 خرداد 1399

چکیده مقاله:

Background & Objective: This study aimed to investigate the effect of decellularized allogeneic bone graft enriched by periosteal stem cells (PSCs) and growth factors on the bone repair process in a rabbit model, which could be used in many orthopedic procedures. Methods: In this experimental study, a critical size defect (CSD) (10 mm) was created in the radial diaphysis of 40 rabbits. In group A, the defect was left intact with no medical intervention. In group B, the defect was filled by a decellularized bone graft. In group C, the defect was implanted by a decellularized bone graft enriched with platelet growth factors. In group D, the defect was treated by a decellularized bone graft seeded by periosteal mesenchymal stem cells (MSCs). Also, in group E, the defect was filled by a decellularized bone graft enriched with platelet growth factors and periosteal MSCs. Radiological evaluation was done on the first day and then in the second, fourth, and eighth weeks after the operation. The specimens were harvested on the 28th and 56th postoperative days and evaluated for histopathological criteria. Results: The radiologic and microscopic analysis of the healing process in bone defects of the treated groups (C, D, and E) revealed more advanced repair criteria than those of groups A and B significantly (P<0.05). Conclusion: Based on this study, it appears that implantation of concentrated PSCs in combination with growth factors and allogeneic cortical bone graft is an effective therapy for the repair of large bone defects.

نویسندگان

Hadi Hassibi

Department of Veterinary Surgery, Shahid Bahonar University of Kerman, Kerman, Iran

Alireza Farsinejad

Department of Hematology and Medical Laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Science, Kerman, Iran

Shahriar Dabiri

Pathology and stem cell Research Center, Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran

Dariush Vosough

Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

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  • Schindeler A, McDonald MM, Bokko P, Little DG. Bone remodeling ...
  • Frolke JP, Patka P. Definition and classification of fracture non-:union:s. ...
  • Killington K, Mafi R, Mafi P, Khan WS. A systemic ...
  • Reed AA, Joyner CJ, Brownlow HC, Simpson AH. Human atrophic ...
  • Bishop JA, Palanca AA, Bellino MJ, Lowenberg DW. Assessment of ...
  • Robinson CM, McQueen MM, Wakefield AE. Estimating the risk of ...
  • Rosenberg GA, Sferra JJ. Treatment strategies for acute fractures and ...
  • Yellowley C. CXCL12/CXCR4 signaling and other recruitment and homing pathways ...
  • Malhotra A, Pelletier MH, Yu Y, Walsh WR. Can Platelet-rich ...
  • Nandi SK, Roy S, Mukherjee P, Kundu B, De DK, ...
  • Brydone AS, Meek D, Maclaine S. Bone grafting, orthopaedic biomaterials, ...
  • Lohmann CH, Andreacchio D, Köster G, Carnes Jr DL, Cochran ...
  • Pokorny JJ, Davids H, Moneim MS. Vascularized bone graft for ...
  • Bigham-Sadegh A, Karimi I, Alebouye M, Shafie-Sarvestani Z, Oryan A. ...
  • Elder BD, Eleswarapu SV, Athanasiou KA. Extraction techniques for the ...
  • Vavken P, Joshi S, Murray MM. TRITON‐X is most effective ...
  • Zhang AY, Bates SJ, Morrow E, Pham H, Pham B, ...
  • Gui L, Chan SA, Breuer CK, Niklason LE. Novel utilization ...
  • Bahney CS, Zondervan RL, Allison P, Theologis A, Ashley JW, ...
  • Debnath S, Yallowitz AR, McCormick J, Lalani S, Zhang T, ...
  • Yang JW, Park HJ, Yoo KH, Chung K, Jung S, ...
  • Janicki P, Schmidmaier G. What should be the characteristics of ...
  • Zimmermann G, Moghaddam A. Allograft bone matrix versus synthetic bone ...
  • Toosi S, Behravan N, Behravan J. Non:union: fractures, messenchymal stem ...
  • Wise DL. Preclinical evaluation of bone graft substitutes. In: Bensen ...
  • Oryan A, Alidadi S, Bigham-Sadegh A, Moshiri A: Healing potential ...
  • Emery SE, Brazinski MS, Koka A, Bensusan JS, Stevenson s. ...
  • Shafiei Z, Bigham AS, Dehghani SN, Nezhad ST: Fresh cortical ...
  • Gugala Z, Lindsey RW, Gogolewski S. New approaches in the ...
  • Sato K, Watanabe Y, Harada N, et al. Establishment of ...
  • Meesters DM, Wijnands KA, Brink PR, Poeze M. Malnutrition and ...
  • Cooper GM, Mooney MP, Gosain AK, Campbell PG, Losee JE, ...
  • Beno T, Yoon YJ, Cowin S, et al. Estimation of ...
  • Pisetsky DS. DNA and the immune system. Ann Intern Med ...
  • Mygind T, StiehlerM, Baatrup A, Li H, Zou X, Flyvbjerg ...
  • Hashimoto Y, Funamoto S, Kimura T, et al. The effect ...
  • Allen MR, Hock JM, Burr DB. Periosteum: biology, regulation, and ...
  • Petite H, Vandamme K, Monfoulet L, Logeart-Avramoglou D. Strategies for ...
  • Mousaei Ghasroldasht M, Matin MM, Kazemi Mehrjerdi H, Naderi-Meshkin H, ...
  • Dozza B, Salamanna F, Baleani M, Giavarei G, Parrilli A, ...
  • Jaiswal N, Haynesworth SE, Caplan AI, et al. Osteogenic differentiation ...
  • Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential ...
  • Granero-Molto F, Weis JA, Miga MI, et al. Regenerative effects ...
  • Undale A, Fraser D, Hefferan T, et al. Induction of ...
  • Dequeker J, Mohan S, Finkelman RD, Aerssens J, Baylink DJ. ...
  • Liu Y, Huse R O, de Groot K, Buser D, ...
  • Schmidmaier G, Wildemann B, Stemberger A, Haas NP, and Raschke ...
  • Arinzeh TL, Peter SJ, Archambault MP, van den Bos C, ...
  • Ding L, Tang S, Liang P, Wang C, Zhou Pf, ...
  • Oryan A, Baghaban Eslaminejad M, Kamali A, Hosseini S, Moshiri ...
  • Chen X, Wang J, Yu L, Zhou J, Zheng D ...
  • Granero-Molto F, Weis JA, Miga MI, et al. Regenerative effects ...
  • Romero R, Travers JK, Asbury E, Pennybaker A, Chubb L, ...
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