Application of radioimmunoassay technique for determination of antigen concentration in different cells with a new monoclonal antibody [Persian]
محل انتشار: مجله پزشکی هسته ای ایران، دوره: 14، شماره: 2
سال انتشار: 1385
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 296
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شناسه ملی سند علمی:
JR_IRJNM-14-2_006
تاریخ نمایه سازی: 27 فروردین 1399
چکیده مقاله:
Introduction: Binding a monoclonal antibody to tumor associated antigens is an effective method for cancer therapy because these agents can specifically target malignant cells, in fact monoclonal antibodies are effective agents for diagnosis, grading and treatment of different kinds of cancers. Methods: In this research, a new monoclonal antibody against colon cancer cells was prepared and antigen concentration in different cells determined by a radioimmunoassay method using iodine (I-125) labeled protein G. Results: 125I-labeled protein G percent binding to white blood cell, HT29, LS180 and MCF7 cell lines were 7.1%, 91.2%, 75.8% and 40.2%, respectively. Conclusion: Regarding importance of monoclonal antibody applications, it is necessary to find an efficient method for their evaluation in cancer therapy. In this method, a radioactive agent with no count restriction was used. Also by this method, amount of the antigen can be easily quantified.
کلیدواژه ها:
نویسندگان
Nakisa Zarrabi-Ahrabi
Science and Research Unit, Islamic Azad University, Tehran, Iran
Shideh Montaser-Kouhsari
Science and Research Unit, Islamic Azad University, Tehran, Iran
Payam Behradkia
Department of Radioisotope, Nuclear Research Center, AOEI, Tehran, Iran
Mohammad Shafiei
Department of Radioisotope, Nuclear Research Center, AOEI, Tehran, Iran