Multiwall carbon nanotube- Fe3O4 Coated Sorbent for Magnetic Solidphase Extraction and High-performance Liquid Chromatography Determination of Phenobarbital in Human Plasma

Magnetic multiwall carbon nanotube was fabricated and successfully applied for magnetic solid phase extraction (MSPE) followed by high-performance liquid chromatography (HPLC) determination of phenobarbital in human plasma. Co-precipitation and electrostatic self-assembly methods were utilized to synthesize magnetic multiwall carbon nanotube. The factors influencing the extraction efficiency such as desorption conditions, pH of sample solution, amount of sorbent, extraction time and salt concentration were evaluated and optimized. The calibration curve showed linearity in the range of 45-1500 ng mL-1 with regression coefficient corresponding to 0.993. Limits of detection (LOD, S/N = 3) and quantification (LOQ, S/N = 10) were 12 and 45 ng mL-1, respectively.

کلیدواژه ها:

Magnetic multi wall carbon nanotube ، high-performance liquid chromatography ، human plasma ، phenobarbital