Cell type-specific Effect of miRZip-21 to Suppress miR-21 in Human Glioma Cell Lines
محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 10، شماره: 2
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 454
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شناسه ملی سند علمی:
JR_JCMR-10-2_002
تاریخ نمایه سازی: 29 اسفند 1398
چکیده مقاله:
There are different subtypes of brain tumors, classified according to the origin of the abnormally proliferatedglial cells. Glioblastoma multiforma (GBM) is the grade 4 of brain tumors, gliomas, with the least life expectancy.microRNAs (miRNAs) are small, single stranded, non-coding RNAs with 20-25 nt length with post-transcriptional generegulatory activities. An altered expression of miRNAs is linked to developmental disorders and some diseases, mostimportantly cancers. miR-21 is a well-known microRNA, overexpressed in almost all cancer types, including braintumors. It targets several genes with vital roles in cellular pathways involve in proliferation, invasion and metastaticbehaviors. Exosomes are 30-100 nm extracellular vesicles which are packed with various molecules, includingmiRNAs. Here, we suppressed miR-21 expression level in HEK-293T cells by transfecting them with the miRZip-21vector. However, when U87-MG cells were cultured in the presence of exosomes isolated from conditioned medium ofengineered HEK-293T cells derived exosomes, we did not observe any suppressing effect on host cells’ miR-21expression level. Moreover, by analyzing the effects of miRZip-21-enriched cell’s conditioned media on three otherbrain cell lines including 1321N1, A-172 and DAOY, cell type-specific effects of exocrine miRZip-21 were revealed.These data suggested that cell lines from different brain tumor subtypes could exert different responses to microRNAbasedtherapies, based on their cellular origin and clinical behaviors.
کلیدواژه ها:
نویسندگان
Hamideh Monfared
Department of Molecular Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Yavar Jahangard
Department of Molecular Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Maryam.M Nikkhah
Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
Seyed Javad Mirnajafi-Zade
Department of Physiology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
Seyed Javad Mowla
Department of Molecular Genetics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran