An invivo study on protective effects of Ziziphus jujube seeds alcoholic extract against rifampicin-induced acute renal-toxicity

Background and Objective: Drugs are important causes of kidney injury. Rifampicin is one of the major anti-tuberculosis drugs and a second choice anti-staphylococcal agent. Renal toxicity of rifampicin has been reported sporadically. The deterioration in renal function typically appears acutely, after reintroduction of rifampicin determined by an acute tubulointerstitial nephritis and acute tubular necrosis. The use of natural remedies for the treatment and prevention of kidney diseases has a long history. Ziziphus jujuba plant is one of local herbs in Iran. In current study we aim to explore the protective effects of this medicinal plant on acute drug-induced renal-toxicity in rats. Materials and Methods: In our interventional study, for preparation of alcoholic extract, washed and soaking jujube and then separating the fruit from the core and washing the core and let it dry under sun light (1 week), then powder it, mix it with 70% methanol and after we shook it with glass splinters put it All night at room temperature. forty healthy male rats (200-–220 g) that were purchased from center for experimental medicine of Birjand university of medical sciences were randomly assigned into 5 groups and in each group contained 8 mice (n =8/per cage). The groups were as follow: 1. Healthy control group 2. Renal toxicity control group Receiving Rifampicin 150 mg/kg 3. Receiving Rifampicin 150 mg/kg with alcoholic Z. jujuba 100 mg/kg, 4. Receiving Rifampicin 150 mg/kg with alcoholic Z. jujuba 200 mg/kg, 5. Receiving Rifampicin 150 mg/kg with Silymarin, Different doses of Z.jujuba (100 and 200 mg/kg/inject) are used for 2 weeks after renal toxicity induced by rifampicin (150 mg/kg/inject). Blood samples were taken at the end of the treatment period from the heart and the kidney tissue of the mice was isolated. Finally, we collected the serum of rats. Then the biomarkers including urea, creatinine and BUN were measured by the auto analyzer unit of valiasr hospital in birjand. Data were analyzed by SPSS15 software, using descriptive statistical test (percentage, mean and standard deviation), chi-square test, fisher’s exact test and ANOVA test, and in the case of abnormal distribution of data from the nonparametric equivalence (kruskal-wallis) were analyzed at a significant level α=0.05 Findings: in our intervention study, data illustrated the urea and creatinine levels notably decreased after Z. Jujube treatment in renalotoxic groups. In addition, the treatment with this herb improved oxidative stress status in renal toxic rats. Conclusion: change in BUN level and other things covered renal-protective effects of this plant. However, more studies required to prove our strategy.

کلیدواژه ها:

Renal-toxicity ، Rifampicin ، Ziziphus jujube