Physical Properties and Stability of Plasmid DNA-Loaded Chitosan-TPP Nanoparticle

  • سال انتشار: 1398
  • محل انتشار: فصلنامه روشهای تصفیه محیط، دوره: 7، شماره: 3
  • کد COI اختصاصی: JR_JETT-7-3_024
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 506
دانلود فایل این مقاله

نویسندگان

S. R. Naeimi Torshizi

Department of cellular and molecular, Nour Danesh Institute of Higher Education, Mymeh, Isfahan

H Ofoghi

Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran

A Jangjou

Department of Emergency Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

S. Taghizadeh

Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran

M Kianirad

Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran

چکیده

Chitosan (CS) is a biodegradable natural polymer that has shown potential for gene delivery. Although a number of in vitro studies showed that chitosan and its derivatives have emerged as promising vehicles for efficient non-viral gene and plasmid DNA (pDNA) vaccine delivery, the stability of chitosan/plasmid nanoparticle remain insufficient. In the present study, ionically crosslinked chitosan nanoparticles were formulated with plasmid DNA using the ionic gelation technique with sodium tripolyphospate (TPP) as a crosslinking agent. We investigate the stability of chitosan/pDNA nanoparticles which was synthesized by this method. Optimization study showed that chitosan to TPP ratios of 1:0.4(w/w) results in the reproducible formation of nanoparticles with good production yields. SEM and DLS analyses revealed a circular shape of the CS/TPP nanoparticles with an average size diameter of 173 nm. The zeta potential of the nanoparticles was + 10.8 mv. In vitro study of pDNA release from CS/TPP nanoparticles revealed no DNA release following incubation of chitosan/pDNA nanoparticles for up to 1 month, in mediums of PBS and acetic acid at pH 4 and pH 7.4. According to the results, ionically crosslinked CS/TPP nanoparticles have the potential to be used as a biocompatible non-viral gene delivery system with strong stability.

کلیدواژه ها

Chitosan, Gene delivery, Nanoparticles, Ionic gelation

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.