Detection of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells of IVF patients as oocyte quality indicator
- سال انتشار: 1398
- محل انتشار: هشتمین کنگره بین المللی و جشنواره دانشجویی طب تولید مثل و سومین کنگره بین المللی ژنتیک تولید مثل
- کد COI اختصاصی: RMED08_065
- زبان مقاله: انگلیسی
- تعداد مشاهده: 409
نویسندگان
Department of Basic Medical Sciences, International Islamic University Malaysia, Malaysia
Department of Basic Medical Sciences, International Islamic University Malaysia, Malaysia
Department Obstetric and Gynecology, International Islamic University Malaysia, Malaysia
چکیده
Background: Nowadays, the criteria for oocyte selection is based on morphological criteria however, it needs further improvement to select the best embryo quality. The gene expression in the cumulus cell plays important role in signaling for follicular development as well as for oocyte quality.Objective: The aim of this study is to investigate the present of HAS2 and GREM1 gene expression in the cumulus cells that can become a useful marker for oocyte quality.Materials and Methods: A case study was performed on cumulus cells derived from four different patients that undergo assisted reproductive technique treatment. Cumulus cells were isolated from 4 IVF patients who have different pathological conditions, oocyte and embryo grading. The expressions of HAS2 and GREM1 were analyzed by using reverse transcriptase polymerase chain reaction (RT-PCR). The products of the PCR were then being quantified and statistical analysis have been done to test the significant of the data.Results: The results showed that HAS2 and GREM1 were expressed differently in each patient. Those genes have been detected in patient 2, 3 and 4 with grade 3 oocytes, whereas, the genes were absent in patient 1 with grade 4 oocytes. The embryo grades and pathological conditions have shown no influence in those genes expression. This showed that the genes expression influenced the oocyte quality.Conclusion: Hence, the measurement of HAS2 and GREM1 expressions in cumulus cells would possibly useful as a tool for selecting competence oocytes with greater chances to be fertilized in assisted reproductive technique.کلیدواژه ها
Cumulus cells, HAS2, GREM1, RT-PCR, oocyte qualityمقالات مرتبط جدید
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