Destructive Effect of Atmospheric O2 Concentration on Blastocyst Quality Parameters During Implantation Time
- سال انتشار: 1397
- محل انتشار: چهارمین کنگره بین المللی تولیدمثل
- کد COI اختصاصی: ISERB04_174
- زبان مقاله: انگلیسی
- تعداد مشاهده: 533
نویسندگان
Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB),
Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB),
Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB),
Department of Animal Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB),
چکیده
IT is generally accepted that quality of embryo which can be largely attributed to its ICM cells and there is a strong link between number of ICM and success of implantation. Previous studies by differential staining method have proven atmospheric O2 concentration during embryo culture due to increasing the free O2 radical’s levels has a destructive effect on ICM cell number, while this parameter is improved when the embryos are cultured in low Oxygen tension. Some transcription factors interaction such asOCT4 and NANOG responsible for early lineage segregation and ICM formation during embryo development. However there are no reports of the low O2 concentration effect on these factors expression in embryo during implantation step. Therefore, in this study effect of hypoxia condition on the expression level of OCT4 and NANOG genes in blastocysts were examined. In this regard, 2-cell embryos from NMRI mice were distributed into hypoxia (5% O2) and atmospheric (20%O2) groups and were cultured into blastocyst stage.Then, the effect of O2 concentration on the genes expression as well as blastocyst formation, hatching and implantation rate were studied. According to our results, blastocyst formation, hatching and implantation rates are improved when the embryos are cultured in hypoxic condition. Furthermore, real-time RT-PCR analysis data showed an increase in the expression level of OCT4 and NANOG in 5% O2 group. In conclusion, it seems that hypoxic condition through increased level of OCT4 and NANOGcould improve ICM cell number and implantation rate and highlight this condition support better embryo development.کلیدواژه ها
OCT4 , NANOG, implantation, ICM, hypoxia, blastocystمقالات مرتبط جدید
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