The effect of small molecule PFT-μ on mouse spermatogonial stem cells apoptosis

  • سال انتشار: 1396
  • محل انتشار: سومین کنگره بین‌المللی تولیدمثل
  • کد COI اختصاصی: ISERB03_358
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 406
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نویسندگان

Sara Moghadasi

National Institute of Genetic Engineering and Biotechnology, Institute of Agricultural Biotechnology, Department of Animal biotechnology

Farid Heidari

National Institute of Genetic Engineering and Biotechnology, Institute of Agricultural Biotechnology, Department of Animal biotechnology

Mehdi Shamsara

National Institute of Genetic Engineering and Biotechnology, Institute of Agricultural Biotechnology, Department of Animal biotechnology

Ehsan Hashemy

National Institute of Genetic Engineering and Biotechnology, Institute of Agricultural Biotechnology, Department of Animal biotechnology

چکیده

Background: Spermatogonial stem cells (SSCs) are responsible for the propagation of a father’s genetic material by spermatogenesis. As well, SSC is intended for the treatment of infertility and other purposes, including correction of inherited disorders. Also SSCs can produce embryonic-like stem cells in vitro which could be used as an alternative for therapeutic, diagnostic cases and therefore, improve techniques to manipulate them in vitro as a prerequisite to achieve the mentioned goals. In this study, we sought to optimize culture conditions to maintain the survival and self-renewal of these cells. Recent studies show that inhibition of p53 has positive effect on the viability in some stem cells.Methods: Germ stem cells extracted from newborn mouse testis then the appropriate concentration PFT-μ, inhibitor of p53 located on mitichondria, determined using the MTT assay and the cells were treated with optimal concentration, then apoptosis was assessed by testing Annexin-V / PI in cells treated with optimal amounts of PFT-μ.Result: The results showed that cell apoptosis at optimal concentrations decreased in compared with the control group.Conclusion: PFTμ by inhibiting of p53 located on mitochondria release of Bcl2 and inhibit stems cell death. So spermatogonial cells apoptosis expected to be reduced in the presence PFTμ .

کلیدواژه ها

Apoptosis, Germ stem cell, Mitochondrial p53 inhibitor, PFT-μ, Spermatogonial stem cell

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