In vitro effects of vitamin E on sperm DNA fragmentation, mitochondrial membrane potential and lipid peroxidation in asthenoteratozoospermic samples
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_245
- زبان مقاله: انگلیسی
- تعداد مشاهده: 354
نویسندگان
Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
Arak Infertility Center, ACECR, Arak, Iran
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran
Arak Infertility Center, ACECR, Arak, Iran
چکیده
Background: In asthenoteratozoospermic men, low motility, defected DNA and highly oxidative stress in sperm cause poor assisted reproductive techniques (ART) outcomes. Vitamin E is one of the most important antioxidative molecules that combat oxidation and prevent oxidative stress. The aim of this study was to determine the effect of vitamin E on sperm quality at different times of in vitro incubation (after 2,4 and 6-h) to improve their semen samples for ART.Methods: Asthenoteratozoospermia semen samples of 30 volunteers were collected in an ethical manner and examined. Each sample was divided into two groups of control and test. In test group, sample treated with 2mM vitamin E and incubated at 37 ˚C for 2, 4 and 6 h. After this incubation sperm motility, viability and plasma membrane integrity, mitochondrial membrane potential (MMP), sperm DNA fragmentation (Tunel and SCD) and malondialdehyde (MDA) levels were evaluated. Data were analyzed using repeated measurement of ANOVA and T-test. The means were considered significantly different at PResult: Vitamin E significantly increased sperm motility, viability, plasma membrane integrity and MMP in asthenoteratozoospermia semen samples compared to untreated control group (PConclusion: In vitro supplementation of vitamin E in asthenoteratozoospermia semen samples can lead to improve sperm parameters, lipid peroxidation and DNA integrityکلیدواژه ها
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