Thymoquinone as a natural spermostatic substance in reproductive medicine: An experimental study
- سال انتشار: 1396
- محل انتشار: مجله طب تولید مثل ایران، دوره: 15، شماره: 10
- کد COI اختصاصی: JR_IJRM-15-10_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 350
نویسندگان
phd.Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
M.Sc. Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
Ph.D Department of Anatomical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده
Background: Nonoxynol-9 a nonionic surfactant is widely used for its spermicidal effects. Finding new sperm immobilizing agents is necessary because Nonoxynol-9 damages the tissues of female reproductive system. Objective: The aim of this study was to evaluate the effects of Thymoquinone (TQ) as a potential spermostatic compound on the motility and viability of human spermatozoa. Materials and Methods: In this experimental study, the effects of 5, 10, 20, 50, 100 μg/ml, 1 and 10 mg/ml of TQ on normozoospermic semen samples were investigated. Sperm motility and viability were compared between untreated and TQ-treated aliquots of each semen sample. To evaluate the effects of TQ on the alteration of mitochondrial membrane potential (MMP), 32 semen samples were examined using 50 μg/ml of TQ. Flow cytometric analysis was performed after staining of spermatozoa with JC-1. Results: Doses above 20 μg/ml of TQ could eventually immobilize all spermatozoa in culture medium. Adding 50 μg/ml of TQ did not significantly diminish the percentage of viable spermatozoa and flow cytometry results revealed that this amount of TQ could decrease sperm MMP. Conclusion: TQ could discontinue the movement of sperm cells in medium without reducing the population of live spermatozoa. It is more likely that TQ exerts its spermostatic action by mitigating the MMP of spermatozoa. Therefore, TQ could be considered as a potential new natural spermostatic chemicalکلیدواژه ها
Nigella sativa, Sperm immobilizing agents, Sperm motility, Thymoquinoneمقالات مرتبط جدید
- رابطه بین جهت گیری ایمان فردی شده و بهزیستی روانشناختی در زنان
- اثربخشی آموزش مهارت های زندگی بر استرس ادراک شده زنان مطلقه
- Investigation of the interaction of tamoxifen drug with p۷۰ S۶ kinase protein using molecular docking analysis
- Stress and women's health: An aspect of its role in the female reproductive system
- تاثیر شیردهی در مادران وابسته به مصرف مواد مخدر بر سندروم محرومیت نوزاد:یک مطالعه مروری
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.