The effects of vitrification on spindle apparatus of in vitro matured germinal vesicle in mice
- سال انتشار: 1387
- محل انتشار: مجله طب تولید مثل ایران، دوره: 6، شماره: 4
- کد COI اختصاصی: JR_IJRM-6-4_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 635
نویسندگان
Department of Anatomy, Faculty of Medicine, Yasouj University of Medical Sciences, Yasuj, Iran.
Department of Anatomy, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan,Iran.
Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background: Routine oocytes cryopreservation remained an elusive technique in the wide ranges of available assisted reproductive technologies. The microtubules ofoocytes are vulnerable to cryoprotectants and thermal change during cryopreservation. Objective: The effects of a vitrification protocol were investigated on the spindle andchromosome configurations of mice oocytes cryopreserved at the germinal vesicle stage.Materials and Methods: Germinal vesicle with cumulus cells were transferred tovitrification solution which was composed of 30% (v/v) ethylene glycol, 18% Ficoll-70 and 0.3 M Sucrose either by single step or in step-wise way. Following vitrification and in vitro maturation (MII), the matured oocytes were immonostained for meiotic spindlesand chromosomes, before visualization using fluorescent microscopy. Results: A statistically significant increase was observed in the survival and maturationrate in step-wise vitrification (88.96% and 71.23% respectively) compared to single step vitrification (70.6% and 62.42% respectively) (p< 0.05). Normal spindle morphology after vitrification-thawing in step-wise vitrification group (77.26%) was higher thansingle step vitrification group (64.24%) but lower than control group (94.75%) (p< 0.05). Conclusion: The results suggest that vitrification with step-wise procedure on mice germinal vesicle oocytes has positive effects on survival and maturation rate and normal spindle configuration compare with single step vitrification procedure.کلیدواژه ها
Vitrification, Germinal vesicle oocyte, Mice, Microtubuleمقالات مرتبط جدید
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