Seroepidemiology and molecular detection of Brucella infectionin Iranian horses: Aprovincial study

  • سال انتشار: 1392
  • محل انتشار: فصلنامه طب دامی ایران، دوره: 7، شماره: 1
  • کد COI اختصاصی: JR_IJVM-7-1_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 485
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نویسندگان

K.H Badiei

Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Sharifiyazdi

Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

M Pourjafar

Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

M Ghane

Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

چکیده

Brucellosis is a febrile zoonotic infectionand has worldwide distribution among humans as well asanimals. Although the seroprevalence of brucellosis in variousanimals has been described in Iran, there is only one report onequine brucellosis in the region. OBJECTIVES: This study wascarried out to determine the seroprevalence of brucellosis inracing clubs and private horse owners in the south of Iran and riskfactors associated with the disease in horses. METHODS: 312randomly selected equine serum samples were investigated forthe presence of antibodies against Brucella genus, using slideagglutination by Rose Bengal plate test (RBPT), serum agglutinationtest (SAT) and 2-mercaptoethanol (2-ME) test, usingwhole cell antigen. PCR assay was also used for detection ofclinically suspected cases. RESULTS: Most seropositive horsesin this study were asymptomatic. The true seroprevalence ofbrucellosis was found to be 9.9, 8 and 7% by RBPT, SAT and 2-mercaptoethanol tests, respectively. All horses with history ofclinical signs (3.2% of all samples) had RBPT, SAT and 2-mercaptoethanol positive results. It was also revealed that age,sex and a history of contact with ruminants had no effect onacquiring the infection in positive cases. In the PCR, one of thethree horses with fistula withers produced amplicon of 450 bpfragment of wbo sequences specific to Brucella spp. field strain.CONCLUSIONS: This study showed the seroprevalence ofbrucellosis in horses of Fars province and it was indicated thatthe PCR assay may be helpful in detection of clinically suspectedhorses

کلیدواژه ها

horse, Brucella infection, PCR

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