Comparison of PCR and bacterial culture methods for diagnosis of dairy cattle's subclinical mastitis caused byStaphylococcus aureus

  • سال انتشار: 1386
  • محل انتشار: فصلنامه تحقیقات دامپزشکی، دوره: 62، شماره: 4
  • کد COI اختصاصی: JR_JVR-62-4_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 526
دانلود فایل این مقاله

نویسندگان

M Ghorbanpoor

Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz -Iran

M Seyfiabad shaponri

Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz -Iran

H Moatamedi

Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz -Iran

M Jamshidian

Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz -Iran

چکیده

To compare PCR and bacterial culture methods for diagnosis of subclinical mastitiscaused by Staphylococcus aureus, 100 milk samples from cattle with subclinical mastitis and 20samples from healthy cattle were collected and tested. The samples were cultured on selectiveblood agar and bacteria were identified by standard methods. DNA extracted from samples wassubjected to PCR reaction with species specific primers and PCR products were analyzed byagarose gel electrophoresis. Based on the PCR results the prevalence of subclinical mastitis dueto S. aureus was 25%. In the bacteriological culture of single milk sampling, S. aureus wasisolated from the same samples being positive in PCR. A correlation of 100% was found betweenPCR and single milk sampling culture method by Mc Nemar test. All of the CMT negativesamples were also negative in culture and PCR methods. The results of this study indicate thatthe PCR reaction is sensitive and specific for diagnosis of S. aureus in subclinical mastitis andcan detect this pathogen in milk samples at species level in few hours.

کلیدواژه ها

Staphylococcus aureus, mastitis, bacterial culture, PCR

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.