Neuroprotective role of Nesfatin-۱ via modulating level of IL-۱βafter cerebral ischemia in rat

سال انتشار: 1401
محل انتشار: پنجمین همایش بین المللی زیست شناسی و علوم زمین
کد COI اختصاصی: BIOLOGY05_078
زبان مقاله: انگلیسی
تعداد مشاهده: 104

نویسندگان

Ph.D of Animal Physiology, Faculty of Science, Ilam University

Department of Physiology, Faculty of Medicine, Physiology Research Center, Iran University ofMedical Sciences, Tehran, Iran.

چکیده

Introduction and Background: Interleukin-۱ beta (IL-۱β) is a key contributor to ischemic brain injuryand it is response increases permeability of the blood–brain, such that exacerbates neuronal cell death andneurological deficits. Brain ischemia-reperfusion (I/R) induces irreversible damages, especially in thehippocampus area. Nesfatin-۱, a recently discovered peptide, protects dopaminergic cells againstneurotoxicity with the anti-inflammatory and anti-apoptotic mechanisms. This study was designed for thefirst time to investigate the protective effects of Nesfatin-۱ on the Interleukin-۱ beta (IL-۱β) protein levelin the hippocampus area in an experimental model of transient global cerebral ischemia.Material and Methods: The male Wistar rats were randomly allocated into ۴ groups (sham, Nesfatin-۱,ischemia-reperfusion, and ischemia-reperfusion+ Nesfatin-۱) (n =۷). The model of cerebral ischemia wasprepared by common carotid arteries occlusion for ۲۰ minutes. Nesfatin-۱ (۲۰ μg/kg) and saline (as avehicle) were injected (intraperitoneally) at the beginning of the reperfusion period. The assessment of theIL-۱β protein level in the hippocampus area was performed by Enzyme-linked immunosorbent assay(ELISA).Results: Nesfatin-۱ significantly reduced the level of IL-۱β in the hippocampal area of ischemic ratstreated by Nesfatin-۱, after ischemia that increased in the ischemia group compared to the sham group.Since the IL-۱β is a proinflammatory cytokine that has been identified as an important mediator ofneuronal cell death in cerebral ischemia.Conclusions:The result demonstrated that nesfatin-۱ might suppress I/R-induced neuroinflammation viareduction IL-۱β protein level. That is the time for a hard outcomes trial of inhibition IL-۱β activation byNesfatin-۱ in cerebral ischemia can be promise a new treatment way.

کلیدواژه ها

Nesfatin-۱, cerebral ischemia, hippocampus, Interleukin-۱ beta

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.