Conjugated PNC-۲۷ peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study
- سال انتشار: 1401
- محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 25، شماره: 10
- کد COI اختصاصی: JR_IJBMS-25-10_011
- زبان مقاله: انگلیسی
- تعداد مشاهده: 316
نویسندگان
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
Departments of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Superparamagnetic iron oxide nanoparticles (SPIONs) have been considered promising non-invasive imaging tools in medicine. However, their high surface energy leads to NPs aggregation, while non-targeted SPIONs can cause cytotoxic effects on normal cells. In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC۲۷ peptide as a double targeting agent throughout early cancer diagnosis.Materials and Methods: Initially, PEI was conjugated to PNC۲۷ with HDM-۲-binding domain. Then, SPIONs were loaded into PEI-PNC۲۷ through the ligand exchange method. The physicochemical characteristics of the synthesized NPs were evaluated. The cytotoxicity and targeting efficiency were assayed against HT-۲۹ and CT-۲۶ cell lines along with NIH-۳t۳ as normal cells by MTT method and Prussian blue staining test, respectively. Results: The mean diameter of synthesized carriers was obtained in the range of ۸۶.۶ – ۱۱۶.۱ nm with a positive charge. According to the cytotoxicity results, the binding and uptake abilities of the PNC۲۷ peptide by cancer cells were significantly higher than that of the NIH-۳t۳ cells. However, the results were indicative of the more toxic impacts of targeted synthesized NPs against CT-۲۶ cancer cell line when being compared with HT-۲۹ cells, which may be caused by the different cytotoxicity mechanisms of NPs. In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-۲ expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.Conclusion: The results indicated the efficiency of targeted PEI-coated SPIONs for cancer diagnostic applications.کلیدواژه ها
B-PEI, Cytotoxicity effect, PNC۲۷ peptide, SPIONs, Targeted cancer diagnosticاطلاعات بیشتر در مورد COI
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