Conjugated PNC-۲۷ peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 169

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شناسه ملی سند علمی:

JR_IJBMS-25-10_011

تاریخ نمایه سازی: 11 مهر 1401

چکیده مقاله:

Objective(s): Superparamagnetic iron oxide nanoparticles (SPIONs) have been considered promising non-invasive imaging tools in medicine. However, their high surface energy leads to NPs aggregation, while non-targeted SPIONs can cause cytotoxic effects on normal cells. In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC۲۷ peptide as a double targeting agent throughout early cancer diagnosis.Materials and Methods: Initially, PEI was conjugated to PNC۲۷ with HDM-۲-binding domain. Then, SPIONs were loaded into PEI-PNC۲۷ through the ligand exchange method. The physicochemical characteristics of the synthesized NPs were evaluated. The cytotoxicity and targeting efficiency were assayed against HT-۲۹ and CT-۲۶ cell lines along with NIH-۳t۳ as normal cells by MTT method and Prussian blue staining test, respectively. Results: The mean diameter of synthesized carriers was obtained in the range of ۸۶.۶ – ۱۱۶.۱ nm with a positive charge. According to the cytotoxicity results, the binding and uptake abilities of the PNC۲۷ peptide by cancer cells were significantly higher than that of the NIH-۳t۳ cells. However, the results were indicative of the more toxic impacts of targeted synthesized NPs against CT-۲۶ cancer cell line when being compared with HT-۲۹ cells, which may be caused by the different cytotoxicity mechanisms of NPs. In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-۲ expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.Conclusion: The results indicated the efficiency of targeted PEI-coated SPIONs for cancer diagnostic applications.

نویسندگان

Reihaneh Rahmani

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Majid Darroudi

Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Mohsen Gharanfoli

Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Jamshidkhan Chamani

Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Mehran Gholamin

Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran

Maryam Hashemi

Departments of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

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  • Zhang Y, Li M, Gao X, Chen Y, Liu T. ...
  • Bakhtiari-Asl F, Divband B, Mesbahi A, Gharehaghaji N. Bimodal magnetic ...
  • Lu C, Han L, Wang J, Wan J, Song G, ...
  • Tomitaka A, Hirukawa A, Yamada T, Morishita S, Takemura Y. ...
  • Subbiahdoss G, Sharifi S, Grijpma DW, Laurent S, van der ...
  • Weissig V, Guzman-Villanueva D. Nanopharmaceuticals (part ۲): Products in the ...
  • Revia RA, Zhang M. Magnetite nanoparticles for cancer diagnosis, treatment, ...
  • Ito A, Shinkai M, Honda H, Kobayashi T. Medical application ...
  • Liu F, Le W, Mei T, Wang T, Chen L, ...
  • Hajesmaeelzadeh F, Shanehsazzadeh S, Grüttner C, Daha FJ, Oghabian MA. ...
  • Mosafer J, Abnous K, Tafaghodi M, Mokhtarzadeh A, Ramezani M. ...
  • Almaki JH, Nasiri R, Idris A, Majid FAA, Salouti M, ...
  • Zia A, Rezaei Aghdam H, Saffari M, Farzaneh J, Pirali ...
  • Mokhtarzadeh A, Parhiz H, Hashemi M, Ayatollahi S, Abnous K, ...
  • Parhiz H, Hashemi M, Hatefi A, Shier WT, Farzad SA, ...
  • Rahmani R, Gharanfoli M, Gholamin M, Darroudi M, Chamani J, ...
  • Rahmani R, Gharanfoli M, Gholamin M, Darroudi M, Chamani J, ...
  • Sarafraz-Yazdi E, Bowne WB, Adler V, Sookraj KA, Wu V, ...
  • Tromsdorf UI, Bigall NC, Kaul MG, Bruns OT, Nikolic MS, ...
  • Luo Y, Yang J, Li J, Yu Z, Zhang G, ...
  • Snyder SL, Sobocinski PZ. An improved ۲, ۴, ۶-trinitrobenzenesulfonic acid ...
  • Meyer M, Philipp A, Oskuee R, Schmidt C, Wagner E. ...
  • Gollwitzer C, Bartczak D, Goenaga-Infante H, Kestens V, Krumrey M, ...
  • Munnier E, Cohen-Jonathan S, Hervé K, Linassier C, Soucé M, ...
  • Laemmli UK. Cleavage of structural proteins during the assembly of ...
  • Silva JM, Zupancic E, Vandermeulen G, Oliveira VG, Salgado A, ...
  • Alibolandi M, Ramezani M, Abnous K, Sadeghi F, Atyabi F, ...
  • Alvarez M, Quezada C, Navarro C, Molina A, Bouvet P, ...
  • Gholami L, Oskuee RK, Tafaghodi M, Farkhani AR, Darroudi M. ...
  • Vu-Quang H, Vinding MS, Nielsen T, Ullisch MG, Nielsen NC, ...
  • Hu Y, Li J, Yang J, Wei P, Luo Y, ...
  • Wankhede M, Bouras A, Kaluzova M, Hadjipanayis CG. Magnetic nanoparticles: ...
  • Shevtsov M, Nikolaev B, Marchenko Y, Yakovleva L, Skvortsov N, ...
  • Shevtsov MA, Nikolaev BP, Ryzhov VA, Yakovleva LY, Dobrodumov AV, ...
  • Shevtsov MA, Nikolaev BP, Yakovleva LY, Marchenko YY, Dobrodumov AV, ...
  • Davitt K, Babcock BD, Fenelus M, Poon CK, Sarkar A, ...
  • Sookraj KA, Bowne WB, Adler V, Sarafraz-Yazdi E, Michl J, ...
  • Zhao M, Beauregard DA, Loizou L, Davletov B, Brindle KM. ...
  • Darban SA, Badiee A, Jaafari MR. PNC۲۷ anticancer peptide as ...
  • Mokhtarzadeh A, Parhiz H, Hashemi M, Abnous K, Ramezani M. ...
  • Rosal R, Pincus MR, Brandt-Rauf PW, Fine RL, Michl J, ...
  • Bychkova AV, Sorokina ON, Kovarski AL, Shapiro AB, Rosenfeld MA. ...
  • Steitz B, Hofmann H, Kamau SW, Hassa PO, Hottiger MO, ...
  • Mulens-Arias V, Rojas JM, Sanz-Ortega L, Portilla Y, Pérez-Yagüe S, ...
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  • National Center for Biotechnology Information (۲۰۲۲). PubChem Compound Summary for ...
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