Evaluation of the effect of polyethyleneimine-based nanoparticles containing scorpion venom (Mesobuthus eupeus) on the growth of Sf۹ cell line

  • سال انتشار: 1400
  • محل انتشار: اولین کنگره ملی رویکردهای نوآورانه در سیستم بیولوژی، داروسازی وصنایع غذایی
  • کد COI اختصاصی: SBPFST01_047
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 508
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نویسندگان

Leila Hosseinzadeh

Department of Medical Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Aref Salehzadeh

Department of Medical Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Sara Soleimani Asl

Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Amirhossein Zahirnia

Department of Medical Entomology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Fatemeh Nouri

Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran

چکیده

Despite the benefits of chemical toxins in killing pests, they are dangerous to organisms and the environment. Examining the mechanism of scorpion peptides, it can be said that scorpion venom is a factor in pest control. In this study, we tried to determine the effect of polyethyleneimine-based nanoparticles in increasing the efficiency of the lethal effect of scorpion venom Mesobuthus eupeus on the Sf۹ cell line. Sf۹ cells were placed in plate wells ۹۶. Then concentrations of ۱۰, ۲۵, and ۳۵ μg/ml scorpion venom and polyethyleneimine-based nanoparticles were added to the Sf۹ cell. The cells were exposed to toxins for ۲۴ hours. Then results were determined using the MTT test. This study showed that with increasing the concentration of PEI derivatives, toxicity in the Sf۹ cell line decreases; also considering the effect of PEI hydrophobicity modification on increasing PEI transmission efficiency (LMW), the ability to transfer M. eupeus scorpion venom at a concentration of ۱۰ μg/ml to Sf۹ cell line with minimal toxicity increases. All PEI derivatives were able to effectively deliver the scorpion venom M. eupeus peptide.

کلیدواژه ها

Mesobuthus eupeus, scorpion venom, polyethyleneimine, nanoparticles, Sf۹ cell line.

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