RED AND NEAR-INFRARED LOW-LEVEL LASER THERAPY ENHANCE SECOND-DEGREE BURN WOUND HEALING IN DIABETIC RATS

Introduction: The disorders of production process and healing associated secretion factors lead to delay in wound healing in diabetic patients. This delay in wound healing can lead to amputation in diabetics. Several methods have been proposed to accelerate wound healing in diabetics, including the use of photobiostimulation or low-level lasers. The main objective of this study was to assess the effect of red and infrared low-level laser in pulsed modes on healing of second degree burn injury in diabetic rats.Method and materials: 36 adult male Wistar rats weighing 250-300 g were randomly divided into 4 groups: control, sham, pulsed red laser and pulsed infrared laser. Diabetes was induced by a single i.p injection of streptozotocin (55 mg/kg). After general anesthesia and back hair shaving of each rat, a second degree burn injury was made. The wounds were treated with a red laser (660 nm) and infrared laser (810 nm) with an average power of 200 mW and a dose of 4 J/cm2 in a 14-day period. At the 3, 7 and 14 days after burn wound induction, the wound surface was measured and Percentage of wound healing was calculated. For histopathological analysis the samples were obtained on days 3, 7 and 14 after burn wound inductionResults: The results showed that the percentage of wound healing in infrared and red laser radiation was significantly higher (p <0.05) than sham group. Also, the effect of pulsed infrared laser was significantly (p <0.05) more than the red laser group. And the percentage of wound healing in this group was approximately the same as the percentage of wound healing in the control group. The induction of diabetes significantly reduced the rate of wound healing in the sham group compared to the control group.Conclusion: The study showed that laser therapy with 78 Hz pulsed infrared light is more effective than red light in healing diabetic wounds. These findings suggest that laser therapy with infrared light can be considered as an alternative to conventional treatments for treating diabetic wounds.

کلیدواژه ها:

Photobiostimulation ، Diabetes Mellitus ، Burn ، Skin ، Wound Healing.