Anticholinesterase activity of neurosteroidal alkaloid from the upside-down jellyfish Cassiopea andromeda venom
- سال انتشار: 1398
- محل انتشار: پانزدهمین همایش سراسری سم شناسی ایران
- کد COI اختصاصی: TOXICOLOGY15_042
- زبان مقاله: انگلیسی
- تعداد مشاهده: 555
نویسندگان
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Marine Biotechnology, The Persian Gulf Research and Studies Center, The Persian Gulf University, Bushehr, Iran
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shaheed Beheshti Medical Sciences University, Tehran, Iran
Department of Marine Biotechnology, The Persian Gulf Research and Studies Center, The Persian Gulf University, Bushehr, Iran
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Research institute, Bushehr University of Medical Sciences, Bushehr, Iran
چکیده
Natural compounds from marine organisms have been rarely studied for their acetylcholinesterase inhibitory activities. The aim of this study was to isolate novel compounds with antiAChE activity from the venom of upside-down jellyfish Cassiopea andromeda Forskål, 1775. The compounds of the fractionated venom on gel filtration chromatography were identified by analyzing gas chromatography–mass spectroscopy data. The structure of the isolated compound that showed the most potent antiAChE activity in a docking study was elucidated by different spectral data, including 1H NMR and 13C NMR. Three compounds, including a neurosteroidal alkaloid androtoxin B, were identified from two venom fractions. This neurosteroidal alkaloid showed strong acetylcholinesterase inhibitory activity (IC50 2.24 ± 0.1 μM) compared with the reference standard, galantamine. The results obtained by a docking study demonstrated that Androtoxin B had close contact with two of the three amino acid residues of the catalytic triad of acetylcholinesterase gorge and was accommodated within a peripheral hydrophobic pocket composed of numerous aromatic site chains. In conclusion, the isolated neurosteroidal alkaloid from Cassiopea andromeda was a potent antiAChE agent with strong binding to both the catalytic and peripheral sites of acetylcholinesterase that correlated well with the experimental data. Further studies are required to determine whether androtoxin B could be a potential treatment for Alzheimer s disease.کلیدواژه ها
Upside-down jellyfish; Antiacetylcholinesterase activity; Androtoxin B; Alzheimer s disease; Neurosteroidal alkaloidمقالات مرتبط جدید
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