The effect of different positively charged silver nanoparticles against bacteria, fungi and mammalian cell line.
- سال انتشار: 1396
- محل انتشار: فصلنامه تحقیقات جاری در داروسازی، دوره: 3، شماره: 2
- کد COI اختصاصی: JR_TIPS-3-2_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 556
نویسندگان
چکیده
The bactericidal efficiency of various positively charged silver nanoparticles has been extensively evaluated in literature, but there is no report on efficacy of various positive charged silver nanoparticles. The goal of this study is to evaluate the role of different positive electrical charge at the surface of silver nanoparticles on antibacterial activity against a panel of microorganisms and their biofilm activities and their cytotoxicity. Four different silver nanoparticles were synthesized by different methods, providing four different electrical surface charges (two ionic liquids (imidazolium and pyridinium) with 12 and 18 alkyl chain length) namely C12Im, C12Py, C18Im and C18Py, respectively. The antibacterial activity of these nanoparticles was tested against gram-positive (i.e., Staphylococcus aureus, Bacillus subtilis), gram-negative (i.e., Escherichia coli and Salmonella typhi) bacteria and Candida albicans as fungi. Disc diffusion and micro-dilution tests were used to evaluate the bactericidal activity of the nanoparticles according to CLSI methods. Also primary cytotoxicity assay of nanosilvers was assessed by MTT test.According to the obtained results, C12Py showed the highest bactericidal activity against all microorganisms tested. C18Im had the least and the C12Im had intermediate antibacterial activity. The most resistant bacteria were Escherichia coli. Different positive surface charge of silver nanoparticles was a significant factor affecting their bactericidal activity. Although the nanoparticles capped with pyridinium and 12 alkyl chains showed the highest level of effectiveness against the organisms tested, the silver nanoparticles capped with imidazolium and 12 alkyl chains were also potent against most bacterial species. Cytotoxicity of the silver nanoparticles was negligible.کلیدواژه ها
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.