Genetic Structure of Salinity Tolerance in Rice at Seedling Stage
- سال انتشار: 1398
- محل انتشار: مجله منابع ژنتیک، دوره: 5، شماره: 1
- کد COI اختصاصی: JR_SGR-5-1_004
- زبان مقاله: انگلیسی
- تعداد مشاهده: 509
نویسندگان
Faculty of Agriculture, Shirvan Higher Eduction Complex, North Khorassan, Iran
Faculty of Agriculture, Shirvan Higher Eduction Complex, North Khorassan, Iran
Department of Plant Production, Faculty of Agricultural and Natural Resources Sciences,Gonbad Kavous University, Gonbad Kavous, Iran
Faculty of Agriculture, Shirvan Higher Eduction Complex, North Khorassan, Iran
چکیده
The Oryza sativa L. F8 population derived from a cross between salt tolerance cv. Ahlemi Tarom and salt sensitive cv. Neda was used in the study. Germinated seeds floated on water for 3 d, and after were transferred to float on Yoshida s nutrient solution for 11 d. two weeks after sowing, the seedling was transferred to nutrient solution containing 51.19 mM NaCl (electrical conductivity 6 dSm-1) for 7 d, then NaCl concentration was increased to 163.8 mM (12 dSm-1) for further 7 d. After this period, the traits were measured. The linkage map was performed using F8 populations, 40 SSR markers, 16 ISSR markers (76 alleles), 2 IRAP markers (7 alleles) and iPBS marker (3 alleles). The map length was 1419 cM with an average distance of 13.07 cM between the 2 adjacent markers. The QTL analysis showed that a total of 73 QTLs were identified that controlled 20 traits under normal and stress conditions. Among the QTLs, qCHLN-8, qSLN-8, qWLN-3, qWLN-9, qLAN-3, qLAN-8 and qLAN-9, qRFWN-1, qRFWN-3b and qRFWN-8, qFBN-7, qRDN-1a and qRDN-3 and qNaKSHN-5 under normal conditions and qSL-8, qLL-1a, qNaR-3, qKSH-1 and qKSH-4 and qNaKSH-4 under salinity stress conditions were identified. There are more than 20% explanations for phenotypic changes in the traits. These QTLs, due to the high percentage of justification after validation, could be a good candidate for selection programs with the help of markers in the population of recombinant lines of rice.کلیدواژه ها
ISSR, QTL mapping, Quantitative Traits Loci Salinity, SSRاطلاعات بیشتر در مورد COI
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